Fig. 4
From: Improving the cytotoxicity of immunotoxins by reducing the affinity of the antibody in acidic pH
32A9 scFv-mPE24 and 42A1 scFv-mPE24 entered into lysosome by similar intracellular trafficking. A Western blot to detect the stability of internalized immunotoxins in A431-GPC3 cells with or without lysosomal inhibitor treatment. B Time-dependent internalization and trafficking of 32A9 and 42A1 scFv-mPE24 in A431-GPC3 cells. 32A9 or 42A1 scFv-mPE24 visualized using anti-PE toxin antibody and Alexa647 labeled secondary antibody (red). Nuclei and lysosomes were stained with DAPI (blue) and lysosome tracer (green), respectively. Arrow indicated co-location of immunotoxin and lysosome