Fig. 3
From: Improving the cytotoxicity of immunotoxins by reducing the affinity of the antibody in acidic pH
32A9 scFv-mPE24 and 42A1 scFv-mPE24 showed different protein stabilities and related free toxin release. A Binding activity of immunotoxin to A431-GPC3 cells. Gray: secondary antibody only as a negative control. B Western blotting was used to detect the stability of the internalized immunotoxins. (C). Statistical analysis of (B). Values represent the mean ± SD, n = 3 individual tests, with *P < 0.05 (paired Student’s t-test). D Western blot to detect the accumulation of free toxin after immunotoxin internalization. E Statistical analysis of (D). Values represent the mean ± SD, n = 3 individual tests, with *P < 0.05 (paired Student’s t-test)