Fig. 1
From: Improving the cytotoxicity of immunotoxins by reducing the affinity of the antibody in acidic pH
GPC3 is a potent target for developing immunotoxin strategies in liver cancer. A The transcriptional expression of GPC3 in liver cancer from The Cancer Genome Atlas Program (TCGA database). n = 369 in the LIHC (liver hepatocellular carcinoma) group, n = 50 in the normal liver tissue group, Values represent the mean ± SD, ***P < 0.0001 (two-tailed Student’s t-test). B Immunohistochemical staining of GPC3 in HCC patient tumor tissues and normal liver tissues. C Flow cytometry was used to detect the cell surface expression of GPC3 in HCC cells. A431 cells and A431-GPC3 cells are shown as negative and positive controls, respectively. D WST assay to detect the cytotoxicity of the anti-GPC3 immunotoxin (HN3-mPE24). Values represent the mean ± SD, n = 3 individual tests, with ***P < 0.001 (two-tailed Student’s t-test)