Fig. 5

METTL3 reduced the mRNA stability of SIRT1 via YTHDF2 depending on the m6A manner. A SIRT1 mRNA level in ESCs detected undergo si-YTHDF1, si-YTHDF2, si-YTHDF3 transfection by RT-qPCR. B Reduction enrichment level of SIRT1 in ESCs after YTHDF2 depleting by RIP-qPCR. C mRNA level of SIRT1 in YTHDF2 knockdown and overexpression by RT-qPCR. SIRT1 mRNA decay analysis at the indicated times after actinomycin D (Act D, 5 μg/ml) treatment in ESCs under YTHDF2 overexpressed D, METTL3 overexpressed E, and both overexpressed F by RT-qPCR. G The protein level of YTHDF2 and SIRT1 in ESCs transfected with si-NC, si-YTHDF2, oe-METTL3, and si-YTHDF2 + oe-METTL3 H and si-NC, oe-YTHDF2, si-METTL3, and oe-YTHDF2 + si-METTL3 by western blotting, using GAPDH as an internal control. I The protein level of METTL3, SIRT1, and FOXo3a in increasing and exhausting METTL3 treated ESCs. J The protein level of SIRT1 and FOXo3a in si-SIRT1 transfected ESCs, using GAPDH as an internal control. All above result were shown in means ± SD, ns, p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001