Table 1 Primers for qRT-PCR
Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
MCL1 | TCTCATTTCTTTTGGTGCCTTTG | TAACTAGCCAGTC(TGTTTTGTC |
XIAP | GTGAATGCTCAGAAAGACAGTATGC | TGTCCACAAGGAACAAAAACGATAG |
BIRC3 | GATGCTGGATAACTGGAAAAGAG | TGAAGAAGGAAAAGTAGGCTGAG |
MDMX | ATTTTCCTTTTCAGGTATGGC | AGGTACTGTTTTCGTTGTTGG |
MDM2 | AAGGGAAGAAACCCAAGACAAAGA | GCACATGTAAAGCAGGCCATAAGA |
CYC | TGATGCCTTTGTTCTTATTGG | TTTATTATGAAGTGTTCCCAGTG |
APAF-1 | ATCTGGGCTTCTGATGAAACTGC | CAACACCCAAGAGTCCCAAACAT |
CASP9 | AGCCAACCCTAGAAAACCTTACC | TCACCAAATCCTCCAGAACCAAT |
cBid | GTCACACGCCGTCCTTGCT | CTGTCCGTTCAGTCCATCCCATT |
BAX | AGGATGCGTCCACCAAGAAGC | GGCAAAGTAGAAAAGGGCGACA |
BCL-XL | GAGAATCACTAACCAGAGACGAGA | GGAGAGAAAGTCAACCACCAGC |
PTEN | TAAGGACCAGAGACAAAAAGGGA | GGCAGACCACAAACTGAGGATT |
CDK6 | TGATCAACTAGGAAAAATCTTGGAC | GGCAACATCTCTAGGCCAGT |
NEK2 | TGTCTCTGGCAAGTAATCCAG | CAGGTCCTTGCACTTGGACT |
CCND1 | AGCTGTGCATCTACACCGAC | TGTGAGGCGGTAGTAGGACA |
CCNE2 | ACCTCATTATTCATTGCTTCCAA | TCTTCACTGCAAGCACCATC |
BIRC5 | TTCTCAGTGGGGCAGTGGATG | TTTCTCAAGGACCACCGCATCT |
CDK4 | CTTCTGCAGTCCACATATGCAACA | CAACTGGTCGGCTTCAGAGTTTC |
P53 | AGCTTTGAGGTGCGTGTTTGTG | TCTCCATCCAGTGGTTTCTTCTTTG |
RB1 | CACAACCCAGCAGTTCAATATC | TGAGATCACCAGATCATCTTCC |
ATM | TGTGACTTTTCAGGGGATTTG | ATAGGAATCAGGGCTTTTGGA |
ATR | GGGAATCACGACTCGCTGAA | CTAGTAGCATAGCTCGACCATGGA |
XAF1 | GCCTACTTGCTGTGGTGGTCTTGT | ACGCCTGGTTTGTTGAGGGTTTT |
P21 | TTAAACAAAAACTAGGCGGTTGA | AGGAGAACACGGGATGAGGA |
CASP3 | TGGCATTGAGACAGACA | GGCACAAAGCGACTG |