Fig. 7

Induction of tumor cell apoptosis by ZBH-01, CPT-11, and SN38 (50 nmol/l, 24 h). A Scatter diagrams of apoptosis of SW1116 cells at different times. B Statistical analysis of A. Annexin V⁺/PI⁻ (%), 12 h: ^*p = 0.0176, ZBH-01 vs. CPT-11. 48 h: ^*p = 0.0284, ZBH-01 vs. CPT-11. 72 h: ^**p = 0.0047, ZBH-01 vs. CPT-11; ^**p = 0.0067, ZBH-01 vs. SN38. ANOVA & two-tailed t-test, n = 3. C Scatter diagrams of apoptosis of LS174T cells at different times. D Statistical analysis of C. Annexin V⁺/PI⁻ (%), 12 h: ^*p = 0.0148, ZBH-01 vs. CPT-11; ^*p = 0.0124, ZBH-01 vs. SN38. 18 h: ^*p = 0.0341, ZBH-01 vs. CPT-11; ^*p = 0.0374, ZBH-01 vs. SN38. 24 h: ^*p = 0.0404, ZBH-01 vs. CPT-11; ^*p = 0.0299, ZBH-01 vs. SN38. 48 h: ^*p = 0.0399, ZBH-01 vs. SN38. 72 h: ^*p = 0.0073, ZBH-01 vs. CPT-11; ^**p = 0.0070, ZBH-01 vs. SN38. ANOVA & two-tailed t-test, n = 3. E Scatter diagrams of MMP of LS174T cells in response to drug treatment (50 nmol/l) at different time points. F Statistical analysis of E. 12 h: ^*p = 0.0178, ZBH-01 vs. SN38. 18 h: ^**p = 0.0047, ZBH-01 vs. CPT-11; ^**p = 0.0047, ZBH-01 vs. SN38. 24 h: ^****p < 0.0001, ZBH-01 vs. CPT-11; ^****p < 0.0001, ZBH-01 vs. SN38. ANOVA & two-tailed t-test, n = 2