Fig. 6

Cell cycle alteration in response to drug treatments. LS174T and SW1116 cells were treated with 50 nmol/l ZBH-01, CPT-11, and SN38 for 12, 18, and 24 h. The percentage of cells in G₀/G₁, S, and G₂/M phases were analyzed by flow cytometry. A Histograms of cell cycle distribution of LS174T cells at different time points. B Statistical analysis of A. 12 h: χ² = 7.5391, p = 0.2738; 18 h: χ² = 40.6417, ^****p < 0.0001; 24 h: χ² = 60.8731, ^****p < 0.0001 C. Histograms of cell cycle distribution of SW1116 cells at different times. D Statistical analysis of C. 12 h: χ² = 96.9852, ^****p < 0.0001; 18 h: χ² = 242.4146, ^****p < 0.0001; 24 h: χ² = 197.4546, ^****p < 0.0001. ANOVA & two-tailed t-test, n = 3