Fig. 5
The proteins encoded by ZBH-01 group-specific DEmRNAs were prominently involved in cell cycle regulation A. The PPI network. The yellow part highlights the most prominent module. B Enlargement of the yellow module in (A), containing 73 genes. C Most significantly enriched KEGG pathway of these 73 genes. D qRT-PCR result showed the relative expression of some DEmRNAs in (B) in LS174T cells after treatment with 50Â nmol/l ZBH-01, CPT-11, and SN38 24Â h (logarithmic transformation). Green: downregulated; Red: upregulated. E, The result of western blot showed that ZBH-01 is more efficient than CPT-11 and SN38 in regulating the expression of three representative cell cycle related genes, MYBL2, CCNA2, and CDK2