Fig. 10

In vivo antitumor activity of ZBH-01 and CPT-11 in colon cancer xenograft model in nude mice. A Tumor weights (g) at the end of the experiment. ***p < 0.0001, CPT-11 vs. control, ***p < 0.0001, ZBH-01 vs. control, **p = 0.0019, ZBH-01 vs. CPT-11. B Body weights (g) at various times. ***p = 0.0001, CPT-11 vs. control, **p = 0.0040, ZBH-01 vs. control, **p = 0.0057, ZBH-01 vs. CPT-11. C Relative tumor volumes at various times. p = 0.0724, CPT-11 vs. control, *p = 0.0380, ZBH-01 vs. control, p = 0.2056, ZBH-01 vs. CPT-11. D Relative tumor proliferation at various time. p = 0.5030, ZBH-01 vs. CPT-11. ANOVA & t-test, two-tailed, n = 6. E The representative diagrams of cell apoptosis in vivo detected by flow cytometry. F Statistical analysis of E. **p = 0.0027, ZBH-01vs. control, *p = 0.0291, CPT-11 vs. control, ANOVA & t-test, two-tailed, n = 6. G Representative H&E staining images of the major organs (Heart, Kidney, Spleen, Liver, and Lung) in the control, CPT-11 and ZBH-01 treatment group. Scale bar = 20 µm