Fig. 1
Comparison of the anti-proliferation effects of ZBH-01, CPT-11, and SN38. A Chemical structural of ZBH-01. B The IC50 values of ZBH-01, CPT-11, and SN38 in colorectal tumor cells and SW620/CPT-11 and LOVO/CPT-11 cells evaluated by MTT or CCK8 assay. C The IC50 values of ZBH-01, CPT-11, and SN38 in non-colorectal tumor cells and HcoEpic and 293 cells evaluated by MTT or CCK8 assay. D The anti-tumorigenesis effects of ZBH-01, CPT-11, and SN38 in LS174T cells evaluated by 3D cell culture. The representative Images were captured by a fluorescence microscope (200 ×). ANOVA & two-tailed t-test, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. SW1116, LS174T, HCT-8, HCT-116, CACO-2, SW620 and LOVO, colon adenocarcinoma. A549 and NCI-H446, non-small cell lung cancer. HeLa, cervical carcinoma. SK-OV-3, ovarian cancer. SAOS-2, osteosarcoma. SGC-7901, gastric adenocarcinoma. 7860, renal carcinoma. K562, chronic myelogenous leukemia. A375, melanoma. MCF-7, breast cancer. SMMC-7721, hepatoma. HcoEpic, human colon mucosal epithelia. HEK293, human embryonic kidney cell