Fig. 6

Circ0001955 is associated with CSCC EMT and responds to mTOR inhibitor treatment A Western blot analysis of the protein levels of NCAPG2 and various EMT markers Snail, E-cadherin, and Vimentin in the indicated cells. B Western blot analysis of the protein levels of AKT/mTOR pathway and NCAPG2 in the indicated cells. C The proliferative abilities of SiHa cells were detected by Edu assays in the indicated cells. Original magnification, × 100. Scale bar, 1000 μm. D The cell cycle progression was analyzed by flow cytometry after being transfected in indicated cells. Each experiment was performed at least three times independently. Rapamycin: 500 nM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s nonsignificant