Fig. 4

NCAPG2 is directly targeted by miR-188-3p and indirectly regulated by circ0001955. A miRGate and TargetScan were utilized to predict mRNAs targeted by miR-188-3p. B Schematic illustration of NCAPG2-WT and NCAPG2-Mut luciferase reporter vectors. C The relative luciferase activities were detected in 293 T cells after transfection with NCAPG2-WT or NCAPG2-Mut and miR-188-3p mimics or miR-NC, respectively. D Relative expression of NCAPG2 in CSCC tissues and adjacent non-tumor tissues (Normal) were detected by qRT-PCR (n = 15). F Representative images of NCAPG2 IHC staining in CSCC tissues and adjacent non-tumor tissues (Normal). Scale bar, 100 μm. G and H The relative expression of NCAPG2 mRNA was detected by qRT-PCR after transfection with indicated vectors. I, J and K NCAPG2 protein expression in SiHa cells transfected with indicated vectors. Data were showed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001,****P <0.0001