Fig. 3

circ0001955 directly binds to miR-188-3p and suppresses miR-188-3p activity. A TargetScan and miRanda were utilized to predict miRNAs targeted by circ0001955. B The miR-188-3p binding site on circ0001955 predicted by miRanda. C and D Relative expression of miR-188-3p in CSCC tissues and adjacent non-tumor tissues (Normal) was determined by qRT-PCR (n = 15). E The relative expression of miR-188-3p was detected by qRT-PCR after transfection with indicated vectors. F Schematic illustration of circ0001955-WT and circ0001955-Mut luciferase reporter vectors. G The relative luciferase activities were detected in 293 T cells after transfection with circ0001955-WT or circ0001955-Mut and miR-188-3p mimics or miR-NC, respectively. H RAP assays showed that AGO2 and miR-188-3p could be pulled down by a biotinylated probe designed for the circ0001955 back-splicing site in SiHa cells. I FISH was performed to observe the cellular location of circ0001955 (red) and miR-188-3p (green) in tissues. Scale bar, 100 μm. Data were showed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, n.s, nonsignificant