Fig. 2

Circ0001955 promotes CSCC cell proliferation, migration and invasion. A Schematic illustration of circ0001955 expression vector and siRNAs. B, C, and D qRT-PCR analysis of circ0001955 and CSNK1G1 expression in CSCC cells transfected with si-NC, si-circ0001955, circ0001955 expression vector or vector. E Edu assays were conducted in cells after transfection with indicated plasmids (magnification, × 100). Scale bar, 1000 μm. F The growth curves of cells transfected with indicated vectors were evaluated by CCK8 assays. G Colony formation assays were executed to detect the proliferation of cells transfected with indicated vectors. H Cell migration capacities were detected by wound healing assays after transfected with indicated vectors. Scale bar, 100 μm. I Cell invasion abilities were determined by transwell assays after transfection. Scale bar, 200 μm. J Apoptosis rate was analyzed by flow cytometry after being transfected with indicated plasmids. K The cell cycle progression was analyzed by flow cytometry after being transfected with indicated plasmids. L Apoptotic cells were assessed by TUNEL after transfected with indicated plasmids. Scale bar, 1000 μm. Data were showed as mean ± SD, **P < 0.01, ***P < 0.001, n.s nonsignificant