Fig. 4

LncRNA MEG3 acted as a sponge of miR-21-5p in NSCLC cells. A The luciferase activity of the WT luc-MEG3 or Mut luc-MEG3 after transfection with miR-21-5p mimics in 95D and H1299 cells. B RT-qPCR analysis of the effects of co-transfection with sh-HNRNPA2B1 and (or) si-MEG3 on miR-21-5p expression in 95D and H1299 cells. C RIP for Ago2 protein was used to assess the endogenous expression of lncRNA MEG3 and miR-21-5p in 95D and H1299 cells. D TCGA cohort analysis of the expression levels of miR-21-5p in pair-matched and non-paired NSCLC tissues. E TCGA and CAARRAY analysis of the association of miR-21-5p expression with the prognosis of NSCLC patients. F Colony formation and G Transwell analysis of the effects of co-transfection with miR-21-5p inhibitor and (or) si-MEG3 on cell proliferation and invasion in 95D and H1299 cells. Data are the means ± SEM of three experiments. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001