Fig. 3
HNRNPA2B1 acted by m6A-dependent modification of lncRNA MEG3 in NSCLC cells. A m6A-lncRNA microarray identification of m6A-modified lncRNAs between sh-HNRNPA2B1 and sh-NC transfected 95D cells. B MeRIP analysis of the effects of HNRNPA2B1 knockdown on the m.6A levels of lncRNA MEG3 in 95D and H1299 cells. C RT-qPCR analysis of the effects of HNRNPA2B1 knockdown or overexpression on the expression levels of lncRNA MEG3 in 95D and H1299 cells. D RIP analysis of the binding between HNRNPA2B1 and lncRNA MEG3 in 95D and H1299 cells. E RT-qPCR analysis of the expression levels of lncRNA MEG3 and its correlation with HNRNPA2B1 in 10 pairs of NSCLC tissues. F GES30219 analysis of the association of lncRNA MEG3 expression with the prognosis of NSCLC patients. G Colony formation and H Transwell assays were used to estimate the effects of co-transfection with sh-HNRNPA2B1 and (or) si-MEG3 on cell proliferation and invasion in 95D and H1299 cells. Data are the means ± SEM of three experiments. *P < 0.05; **P < 0.01. ****P < 0.0001