Fig. 5

Mitochondrial abnormalities were involved in the inhibitory effect of ALT against OSCC cells. A, B ATP production in the indicated groups. 10 µM ALT was used to treat CAL27 cells, and 4 µM ALT was added to SCC9 cells. C–F Representative images showing MitoSOX staining and quantification in the indicated groups. 12 µM ALT was used to treat CAL27 cells, and 8 µM ALT was added to SCC9 cells. Scale bar = 100 µm. G–J Representative images showing TMRM staining and quantification in the indicated groups. 12 µM ALT was used to treat CAL27 cells, and 8 µM ALT was added to SCC9 cells. Scale bar = 100 µm. (***p < 0.001, ****p < 0.0001 versus the Control group, ^#p < 0.05, ^###p < 0.001, ^####p < 0.0001 versus the ALT group)