Fig. 7

IL-17A are mainly producing by γδT cells and promotes CD8 + T cells infiltrating the tumour A-B The process of mouse-derived allograft colon cancer models; C H&E staining and immunostaining of CD8 from IL-17A or vehicle-treated colon tumour tissue. 3 fields under 40 × magnification were randomly selected, then counting and statistics CD8-positive cells, data conforms to normal distribution, and tested by two independent samples t-test; D Left panel representative flow cytometric analysis of all CD3 + cells from CD8 + T cells (Tc, top), CD4 + T cells (Th, middle), and γδTCR + T cells (γδT, bottom) and the right panel representative flow cytometric analysis of all CD3 + cells and IL-17A secretion from CD8 + T cells (Tc17, top), CD4 + T cells (Th17, middle), and γδTCR + T cells (γδT17, bottom) in tumour; E Paraffin sections from CRC patients were stained with anti-human pan-γδTCR (green) and anti-human IL-17A (red) for immunofluorescent (IF) staining