Fig. 1
From: Pharmacological targeting of one‑carbon metabolism as a novel therapeutic strategy for glioblastoma
A IC50 measurement of LY345899 and DS44960156. B LN229 and U251 cells were treated with two types of MTHFD2 inhibitors and DMSO for 24 h, which was changed to medium with or without glutamine on Day 1. The cell number over time represents the mean ± SD of four independent experiments (statistically significant with ***p < 0.001). C and D Flow cytometric analysis showing the impact of LY345899 and DS44960156 on apoptosis of LN229 and U251 cells with or without glutamine. The right panels show quantification of the apoptosis rate (statistically significant with ***p < 0.001). E The NAD + /NADH ratio in LN229 and U251 cells treated with MTHFD2 inhibitors. Data represent the mean ± SD of three independent experiments (statistically significant with ***p < 0.001). F Representative fluorescence microscopy images of ROS signals in LN229 and U251 cells. Scale bar: 100 μm