Fig. 6

P21-mediated CCL7 release from injured Beas-2B cells promoted U937 macrophage chemotaxis. A RNA expression levels of some chemokines in Beas-2B cells after IR and/or BLM treatment. B RNA expression levels of chemokines in the irradiated Beas-2B cells with/without p21-siRNA interference. C RNA expression level of CCL7 in p21-siRNA transfected Beas-2B cells after IR and/or BLM treatment. D CCL7 concentration in the supernatants of Beas-2B at 24 h after the treatment of IR and/or BLM. E–F The correlation between CCL7 gene expression level and macrophage infiltration in lung adenocarcinoma (LUAD) in databases integrated by Timer2.0 E and the specifics in the XCELL database F. G-H Representative images G and the number H of migrated U937 cells cultured in a medium containing different concentrations of active CCL7 protein. Scale bar = 50 μm. I qRT-PCR assay of CCL7 in Beas-2B cells after CCL7-siRNA transfection. J The expression level of CCL7 mRNA in Beas-2B cells treated with IR and/or BLM after CCL7-siRNA transfection. K-L Representative images K and the number L of migrated U937 cells after 48 h treatment of CM from CCL7-siRNA transfected Beas-2B cells treated with IR and/or BLM. Scale bar = 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001