Fig. 5

Knockdown p21 in Beas-2B cells reduced the chemotaxis of U937 macrophages. A–B Representative images A and number B of migrated U937 cells after 24 h co-culture with Beas-2B cells treated with IR, BLM, or BLM + IR. Scale bar = 50 μm. C A scheme of the conditional medium (CM) generation. At 24 h after the treatment of IR and/or BLM, the supernatant of Beas-2B cells was collected as CM. D-E Representative images D and the number E of migrated U937 cells after 48 h treatment of the CM with respect to IR and/or BLM treatment. Scale bar = 50 μm. F-G Western blot assay of P21 in Beas-2B cells after p21-siRNA transfection. H The expression level of p21 mRNA in Beas-2B cells treated with IR and/or BLM. I–J Representative images I and the number J of migrated U937 cells after 48 h treatment of CM from p21-siRNA transfected Beas-2B cells treated with IR and/or BLM. Scale bar = 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001