Fig. 2
Functional enrichment analysis of DEGs in the BIPF + RILI model mice. A The schedule for the establishment of BIPF + RILI mouse model. B H&E and Masson’s staining images (left) and the score of lung injury (right) at week 1 and week 4 after irradiation. n = 5, scale bar = 50 μm, magnifying scale bar = 25 μm; **p < 0.01, ***p < 0.0001, ns: no significant. C Volcano plot of the genes in RNA-seq data from BIPF + RILI model at week 1 after irradiation. Red dots represent the upregulated genes (up), blue dots represent the downregulated genes (down), and gray dots represent the genes no changes (ns) in both BLM-instillation and irradiated samples. Adjusted p-value < 0.05 with the threshold |log2(Fold Change)|> 0.585 is considered significant. D Heatmap of the top fifty DEGs between BIPF + RILI model and its control with expression value normalized from – 1–1. E GSEA enrichment pathways of RNA-seq data. F Bar plot showing the top fifteen GOBP pathways enriched by DEGs of RNA-seq data