Fig. 5

The PI3K-Akt-mTOR pathway influences TGF-β1-induced PMT by regulating HKII. A Immunofluorescence staining of HKII (green) and nuclei (blue) and quantitative data with or without inhibition of the PI3K-Akt-mTOR pathway. B Gene expression of HKII with or without inhibition of the PI3K-Akt-mTOR pathway. C HK activity with or without inhibition of the PI3K-Akt-mTOR pathway. D, E HKII rescue experiments showed that HKII overexpression rescued PMT induced by TGF-β1. F LY294002 decreased the mRNA expression of HKII. Scale bar = 50 μm. Data are presented as the mean ± SD (n = 3–8)