Fig. 5

Gating and quantification of immune cells in murine hearts and blood at 4 weeks after sham or AB surgery. A Gating strategy for flow cytometric analysis of the leukocyte populations isolated from the blood of WT and OSMR-KO mice at 4 weeks after sham or AB surgery. Flow cytometry-based quantification of the percentage of Ly6C^high or Ly6C^low cells relative to the total monocyte/macrophage (Mo/MΦ) population and the percentage of F4/80⁺Ly6C^high cells relative to the total Mo/MΦ population in WT and OSMR-KO mice at 4 weeks after sham or AB surgery (n = 4 per experimental group). B Gating strategy for flow cytometric analysis of the leukocyte populations isolated from the hearts of WT and OSMR-KO mice at 4 weeks after sham or AB surgery. Flow cytometry-based quantification of the percentage of Ly6C^high or Ly6C^low cells relative to the total Mo/MΦ population in WT and OSMR-KO mice at 4 weeks after sham or AB surgery (n = 4 per experimental group). Data are presented as the mean ± SEM