Fig. 3

Knockdown of NF1 led to impairment of proliferation of GBC in vivo. A and B The mRNA and protein expression level of NF1 in NOZ cells transfected with lv-Con, lv -shNF1-1, and lv -shNF1-2. C The nude mice used for tumor growth in vivo were shown. D and E Tumor weight was measured after mice were sacrificed and the tumors were resected. Compared with lv -Con groups, knockdown of NF1 suppressed the tumor growth in nude mice. *** P < 0.001 F Tumor growth curves were plotted. G The expression of Ki67 and TUNEL by IHC staining