Fig. 7
α-Actinin-2 promotes primary tumor growth and BMM in vivo. A, B 2 × 105 luciferase-tagged and GFP or GFP-α-Actinin-2 transfected AGS cells were injected into the left cardiac ventricle of anesthetized female nude mice. Bioluminescence images show representative mice from each experimental group (A). Normalized BMM bioluminescence signals from mice (n = 7, B). The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. *P < 0.05 using Ordinary one-way ANOVA. C, D 2 × 106 luciferase-tagged and GFP or GFP-α-Actinin-2 transfected AGS cells were injected into the abdominal cavity of anesthetized female nude mice. Bioluminescence images show representative mice from each experimental group (C). Normalized BMM bioluminescence signals from mice (n = 11, D). E–H TRAP staining of femora. Representative images of cardiac injection and intraperitoneal injection mice. TRAP staining positive cells were indicated by black arrows. (E, F). Quantification of the number of TRAP staining positive cells in cardiac injection and intraperitoneal injection groups (G, H). The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. *P < 0.05, **p < 0.01 using Student’s t-test. I Extracting mouse bone marrow cells from the site of bone metastasis, then the mouse bone marrow cells containing metastatic GFP-α-Actinin-2 overexpressed cancer cells or mouse bone marrow cells + GFP overexpressed AGS cells were lysed for co-Immunoprecipitation (IP) assay by using GFP antibodies