Fig. 2

α-Actinin-2 promotes GC cell migration by facilitating the increase and growth of filopodia. A and C AGS cells were transfected with GFP or GFP-α-Actinin-2 for 24 h, then cells were stained for quantification of the percentage of cells display filopodia, the number of filopodia per cell or the average length of filopodia. B Tissue and cell lysates were harvested and subjected to Western blotting using indicated antibodies, the sampling amount of the second band is one-third of that of the first band. D AGS cells were transfected with GFP-α-Actinin-2 for 2 days following transfect with negative control (NC) siRNAs or α-Actinin-2 siRNAs for 24 h, then cells were stained for quantification of the number of filopodia per cell or the average length of filopodia. The bars indicate the SD. The results are expressed as the mean ± SD of three independent experiments. *P < 0.05 using Student’s t-test for (A). *P < 0.05 using Ordinary one-way ANOVA for (D). GFP/GFP-α-Actinin-2 (green), F-Actin (red) and Nucleus (DAPI, blue). Scale bar, 10 μm