Fig. 1

ACTN2 is a gene associated with BMM in patients with GC. A Whole-body maximum-intensity projection PET image demonstrates the location and high ¹⁸F-fluorodeoxyglucose activity of the tumor in NAGC patient and HAGC patient. B 23 genes overexpressed between adjacent normal tissues and tumor samples of HAGC, 11 genes overexpressed between adjacent normal tissues and tumor samples of NAGC. The label in the upper right corner of the figure, each different color represents a different patient. There were four patients in the HAGC group and five patients in the NAGC group. C The enriched (count ≥ 5) gene ontology terms of up-regulated differentially expressed genes (DEGs) of HAGC patients compared to NAGC patients. D ADIPOQ, FGL1, SPINK6, ACTN2 and SYT12 are specifically upregulated in HAGC but not in NAGC or in The Human Protein Atlas dataset. E and F GC cells invasion and migration after 24 h ADIPOQ, FGL1, SPINK6, ACTN2 and SYT12 transfection were examined by E transwell chamber assays or F wound healing assays. The number of invading tumor cells was counted using 5 high-intensity fields. G 2D single cell migration assessment of GFP and OE-ACTN2 cells (scale bar 10 μm). The bars indicate the SD. The results are expressed as the mean ± SD of five independent experiments. *P < 0.05, ***P < 0.001, ****P < 0.0001, using Student’s t-test