Fig. 4

Analysis of the mean nearest neighbor distance from intratumoral CD8 + T cell to neighboring cells. a Illustration of the calculation of the mNND. b Discrepancies of the mNND from CD8 + T cells to functional subsets between patients with LNM and without LNM. Significance (P value) was determined by Mann–Whitney U test. c–l Representative multiplex immunofluorescence images exhibited the discrepancies between NSCLC patients with and without LNM. Scale bar, 50 mm