Fig. 3
Comparison of the density of intratumoral CD8 + T cells between NSCLC patients with and without LNM. Discrepancies of the densities of CD8 + Ttotal cells (a), CD8 + Tpredys cells (b), CD8 + Tdys cells (c) between NSCLC patients with and without LNM. Significance (P value) was determined by Mann–Whitney U test. d–f Representative multiplex immunofluorescence images (left) and corresponding phenotype map (right) exhibited the discrepancies correspondingly. g Forest plots of univariate and multivariate logistic models demonstrated the relationships between the density of CD8 + T-cell functional subsets and LNM. aThe multivariate logistic regression model adjusted age (≤ 60 years vs. > 60 years), gender (male vs. female), histology subtype (squamous cell carcinoma vs. adenocarcinoma) and tumor diameter (≤ 3 cm vs. > 3 cm)