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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: LINC00963-FOSB-mediated transcription activation of UBE3C enhances radioresistance of breast cancer cells by inducing ubiquitination-dependent protein degradation of TP73

Fig. 4

UBE3C binds to TP73 and inhibits the protein stability. A proteins that can bind to UBE3C predicted via Ubibrowser; B protein level of TP73 in the complexes pulled down by anti-UBE3C examined by WB analysis; C, D mRNA and protein levels of TP73 in parental and radioresistant MDA-MB-231 and SK-BR-3 cells analyzed by RT-qPCR and WB analyses; E ubiquitination level of TP73 in MG132-treated MDA-MB-231 and SK-BR-3 cells; F, binding of UBE3C with TP73 in MDA-MB-231 and SK-BR-3 cells determined by double-label immunofluorescence staining; G the effect of UBE3C on TP73 ubiquitination modification analyzed by the in vitro ubiquitination assay. Data are expressed as the mean ± SD. Three repetitions were performed. Differences were analyzed by two-way ANOVA (B–G). **p < 0.01, ***p < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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