Fig. 6

Overexpression of FSCN1 rescues the function in SYTL2-knockdown cells. A The levels of SYTL2 and FSCN1 protein were detected by Western blotting in SYTL2-knockdown cells transfected with FSCN1. B, C Representative images of cell migration and invasion (B) were analyzed using SYTL2-knockdown or control cells combined with FSCN1 transfection, and a quantification analysis of the migrated or invaded cell counts (C) is shown. D Representative images of wound-healing assays using SYTL2-knockdown or control cells combined with FSCN1 transfected in PC-3 cells, showing rescuing cell motility after using FSCN1 plasmid in SYTL2-knockdown cells (left panel) and a quantification analysis of cell migration index is shown (right panel). E Representative images of three-dimensional (3D) cell culture using SYTL2-knockdown or control cells combined with FSCN1 transfection showing rescued cell motility after using FSCN1 plasmid in SYTL2-knockdown cells; *p < 0.05, **p < 0.01 and ***p < 0.001. Two-way ANOVA was performed to analyze factorial designed data, ^#p < 0.05. Scale bars: 10 μm (red)