Fig. 5
Short-term inhibition of PP2A by Okadaic acid drives changes in gene and protein expression in Panc-1 cells. A Relative gene expression of selected EMT and PP2A genes in Panc-1 cells treated with OA for 24 h vs untreated control cells (GSE114288) (2log values). B Western blot analysis of Panc-1 cells exposed to 20 nM of OA for 24, 48 or 72 h. Expression is shown for vinculin (loading control), PP2A-C, Snail and Epcam. A representative blot is shown. C Protein bands were quantified and relative changes in protein expression were determined relative to vinculin for three independent immunoblot experiments. Average values for n = 3 are displayed; p-values < 0.05 were considered significant (*, two-way ANOVA). D Average percentage viability of Panc-1 cells treated with ethanol (mock) or 20 nM OA for 24 h, 48 h or 72 h as measured with trypan-blue on an automated cell counter (n = 3). p-values < 0.05 were considered significant (two-way ANOVA, ****p < 0.0001)