Fig. 3
89Zr-oxine radiolabeling did not increase apoptosis in CAR-T Cells. A Number of apoptotic cells were counted in radiolabeled and unlabeled CAR-Jurkat and CAR-T cells on day 3 and day 7 as described in Materials and Methods. Each value represents a mean ± SD of three independent experiments performed in quadruplicate. B Caspase 3/7 activities from cell lysates obtained from unlabeled and radiolabeled CAR-Jurkat and CAR-T cells were measured by fluorometric assay on day 3 and day 7. Each value represents a mean ± SD of three independent experiments performed in quadruplicate. C Granzyme B activities from cell lysates obtained from unlabeled and radiolabeled CAR-Jurkat and CAR-T cells were measured by fluorometric assay on day 3 and day 7. Each value represents a mean ± SD of three independent experiments performed in quadruplicate