Fig. 1
Radiolabeling of CAR-T cells with 89Zr-oxine A Effect of temperature on radiolabeling of CAR-Jurkat and CAR-T cells ranging from 4 °C to 37 °C. B Optimal time for radiolabeling of CAR-Jurkat and CAR-T cells performed at room temperature. C 1 × 106 cells were radiolabeled with 89Zr-oxine in PBS, RPMI, RPMI with 10% FBS, T cell medium or T cell medium with IL-2 at room temperature. D 89Zr-oxine radiolabeled CAR-Jurkat and CAR-T cells retained cell associated radiolabel in a cell number dependent manner. Three independent experiments were performed in triplicate and results are expressed as mean ± SD