Fig. 6

CircEPHA3 inhibits the migration and invasion of PCa cells via miR-513a-3p/BMP2. A Up-regulation of circEPHA3 abolished the cell proliferative capacity of PC-3 and DU145 cells enhanced by miR-513a-3p mimics. B, E The migration capability of PC-3 and DU145 cells transfected with miR-513a-3p mimics was counteracted by circEPHA3 in wound healing assays. Scale bars = 100 μm. C, D The migration and invasion capabilities of PC-3 and DU145 cells transfected with miR-513a-3p mimics was neutralized by circEPHA3 in transwell assays. Scale bars = 100 μm. F The western blot showed that circEPHA3 could reverse the effect of miR-513a-3p on BMP2 protein expression in PC-3 and DU145 cells. G, H The migration capability of circEPHA3-overexpressed PC-3 and DU145 cells co-transfected with miR-513a-3p mimics and/or BMP2 siRNA in wound healing assays. Scale bars = 100 μm. I, J The migration and invasion capabilities of PC-3 and DU145 cells co-transfected with miR-513a-3p mimics and/or BMP2 siRNA in transwell assays. Scale bars = 100 μm. K The western blot showed the level of BMP2 protein in PC-3 and DU145 cells that co-transfected with circEPHA3, miR-513a-3p and/or BMP2 siRNA. *, ** and *** represented P < 0.05, 0.01 and 0.001, respectively