Fig. 5

BMP2 is the downstream target of miR-513a-3p and promotes proliferation, migration and invasion of PCa cells in vitro. A, B The western blot showed that miR-513a-3p could down-regulate BMP2 protein expression in PC-3 and DU145 cells. C The schematic of BMP2 wild-type and mutant luciferase reporter vectors. D Relative luciferase activity of HEK-293T cells co-transfected with miR-513a-3p mimics or mimics NC, pmirGlo-wild type BMP2 (BMP2-wt) or pmirGlo-mutant type BMP2 (BMP2-mut) plasmids. E, F The proliferation ability of PC-3 and DU145 cells transfected with BMP2 siRNAs or negative control was evaluated by CCK-8 assay. G, I The migration capability of PC-3 and DU145 cells transfected with BMP2 siRNAs or negative control was measured by wound healing assays. Scale bars = 100 μm. H, J–K The migration and invasion capabilities of PC-3 and DU145 cells transfected with BMP2 siRNAs or negative control were assessed by transwell migration and invasion assays. Scale bars = 100 μm. *, ** and *** represented P < 0.05, 0.01 and 0.001, respectively