Fig. 1

The identification and characterization of circEPHA3 in PCa. A Heat map of differentially expressed circRNA in high-grade and low-grade PCa tissues. (red: upregulation; green: downregulation; h-PCa: high-grade PCa; l-PCa: low-grade PCa). B Relative expression of circEPHA3 in various PCa cell lines. C The existence of circEPHA3 in PC-3 and DU145 cells. D The formation of circEPHA3 was confirmed by Sanger sequencing. E Relative abundance of circEPHA3 and EPHA3 mRNA in PC-3 and DU145 cells exposed to Actinomycin D. F Relative abundance of circEPHA3 and EPHA3 mRNA in PC-3 and DU145 cells in RNase R assay. G The subcellular localization of circEPHA3 in PCa cells was detected by FISH. Scale bars = 10 μm. H Relative integral optical density (IOD) of circEPHA3 in PCa cell. *, ** and *** represented P < 0.05, 0.01 and 0.001, respectively