Fig. 5

Loss of USP37 leads to accumulation of spontaneous replication stress. A U2OS osteosarcoma control cells and cells depleted of USP37 were stained with Anti γH2Ax antibody to assess replication stress after 48 h. B U2OS osteosarcoma control cells and cells depleted of USP37 were treated with 300 µM HU for 24 h and stained with Anti γH2Ax antibody to assess DNA damage response. C U2OS osteosarcoma control cells and cells depleted of USP37 were stained with Anti γH2Ax antibody to assess replication stress after 48 h. D U2OS osteosarcoma control cells and cells depleted of USP37 were treated with cisplatin and stained with Anti γH2Ax antibody to assess DNA damage response. E U2OS osteosarcoma cells were transfected by Myc USP37 plasmid and were treated with 300 µM HU for 24 h. and stained with i. Anti γH2AX, ii. Anti 53BP1iii. Anti RPA. Anti USP37 antibody to assess early DNA damage response in cells having a high and low expression of USP37. Blue arrows are cells with low expression of USP37, while orange arrows are cells with high expression of USP37