Fig. 4

The attenuated oxidative stress by NRP-1 overexpression after ischemia/reperfusion injury. A Expression levels of MDA, B SOD, C GSH/GSSG in the neurons of different groups; n = 3. Data are expressed as mean ± SEM. and analyzed by one-way analysis of ANOVA with Bonferroni’s post hoc test. ***p < 0.001, as compared with the Cont group; ^#p < 0.05, ^###p < 0.001 as compared with the OGD/R + LV-vector group. D Expression levels of GSH-Px in the cortex of different groups; n = 3. Data are expressed as mean ± SEM. and analyzed by one-way analysis of ANOVA with Bonferroni’s post hoc test. ***p < 0.001, as compared with the Sham group; ^##p < 0.01 as compared with the tMCAO + AAV-empty group. E Representative images of DHE staining. F Quantitative determination of fluorescence intensity in cultured neurons of different groups; n = 3. Data are expressed as mean ± SEM. and analyzed by one-way analysis of ANOVA with Bonferroni’s post hoc test. **p < 0.01, as compared with the Cont group; ^#p < 0.05, as compared with the OGD/R + LV-vector group. Scale bar: 100 μm. (Cont = Control)