Fig. 6

Identification of CRC progression-associated differential pathway and its correlation with CRC progression-associated differential intestinal flora. A GO volcano plot of the associated differential expression in the stage I-II CRC versus the stage III-IV CRC. B KEGG Volcano plot of the associated differential expression in stage I-II CRC vs. stage III-IV CRC. The horizontal coordinate indicates log2 (fold change). The farther the dot is from the center, the greater the fold of difference; the vertical coordinate indicates -log10 (p value). The closer to the top the point indicates, the more significant the difference in expression. Each dot represents a detected differentially expressed gene. Red indicates up-regulated genes. Blue indicates down-regulated genes. And gray indicates no differential genes. C Correlation graph of BP and MF of CRC progression-related differences with CRC progression-related differential intestinal microbiome. The horizontal coordinates indicate bacteria. The vertical coordinates indicate GO labels. The red color in the graph represents positive correlation. The blue color represents negative correlation. The color depth represents Spearman correlation coefficient size. The color from light to dark indicates Spearman correlation coefficient value from small to large. The " × " in the graph indicates the size of P-value: with × means P-value ≥ 0.05, without × means P < 0.05. D Correlation graph of CRC progression-related differences in KEGG and CRC progression-related differential intestinal flora. The triangle graph represents the correlation graph of CRC progression-related differential intestinal flora. The upper right corner is the KEGG label. Red font indicates the KEGG pathway upregulated in the stage III-IV group. Blue font indicates the KEGG pathway upregulated in the stage I-II group. The depth of the connecting color represents the magnitude of Spearman's correlation coefficient “r”. The connecting line presented in dark orange indicates the correlation coefficient r ≥ 0.05, representing a strong positive correlation. The connecting line presented in light orange indicates the correlation coefficient 0 < r < 0.05, representing a weak positive correlation. A dark orange line indicates a strong positive correlation; a light orange line indicates a correlation coefficient of 0 < r < 0.05, which represents a weak positive correlation. A dark green line indicates a correlation coefficient r ≥ 0.05, representing a strong negative correlation; a light green line indicates a correlation coefficient 0 < r < 0.05, representing a weak negative correlation; the thickness of the line represents the p-value, with a thick line representing 0.01 < P < 0.05 and a thin line representing P ≥ 0.05