Fig. 2

Differential analysis of intestinal flora of CRC patients in stage I-II group and stage III-IV group. A cladogram by LEfSe analysis. Node size represents species abundance size, which is proportional to species abundance size. Node color represents grouping, yellow nodes in the tree branch indicate species with no significant abundance differences between groups; red nodes indicate species with significantly higher abundance in stage III-IV groups, and green nodes indicate species with significantly higher abundance in stage I-II groups. Each layer of nodes indicates phylum/class/order/family/genus/species from inside-out, and the annotation of each layer of species markers indicates phylum/class/order/family/genus/species from outside-in. B LDA bar graph based on 16S rRNA gene sequencing. The color of the bar represents the group, the horizontal coordinate is the LDA score (after log10 treatment), the vertical coordinate indicates the species with significantly higher abundance in the group, and the length of the bar represents the size of the LDA score value. C Network diagram of the correlation of differential intestinal flora associated with phase I-II groups and phase III-IV groups. Each node represents each species, node color represents grouping, node size represents the number of edges connected to the node, the larger the node, the more the number of edges connected to the node, the connecting line represents the existence of significant correlation between two nodes, Spearman correlation coefficient value below 0 (negative correlation) indicates blue line, Spearman correlation coefficient value greater than 0 (positive correlation) indicates red line The thicker the line, the greater the Spearman correlation coefficient between the two nodes