Fig. 3

STIL regulates PC formation and the SHH pathway. A Western blot analysis for the protein expression (STIL and acetylated tubulin) in SV-HUC-1, BIU87, EJ, T24, and 5637. The percentage of protein levels was quantification. B The protein expression levels of STIL, acetylated tubulin, and ARL13B in STIL-knockdown and control EJ cells. The percentage of protein levels was quantification. C, D The STIL knockdown/overexpression and corresponding control EJ cells were serum-starved for 48 h, then fixed and stained antibodies against ace-tubulin (red) and DAPI (blue) for immunofluorescence analysis. The percentage of ciliated cells were compared between the control and treatment group. E Western blot analysis for the protein expression (including STIL, IFT88, acetylated tubulin, ARL13B, SHH, SMO, and GLI1) in STIL-knockdown and control EJ cells. The percentage of protein levels was quantification. F Western blot analysis for the protein expression (including STIL, IFT88, acetylated tubulin, ARL13B, SHH, SMO, and GLI1) in STIL-overexpression and control EJ cells. The percentage of protein levels was quantification. G Control and STIL overexpression EJ cells were fixed and stained with antibodies against GLI1 for immunofluorenscence analysis. H, I siNC and siSTIL EJ cells transfected with siIFT88 or not, respectively, were serum-starved for 48 h, then fixed and stained with antibodies against ace-tubulin (red) and DAPI (blue) for immunofluorescence analysis. The scale bar indicates 10 μm. The percentage of ciliated cells was quantification. J, K The protein expression levels (including STIL, acetylated tubulin, ARL13B, IFT88, SHH, and GLI1) in siNC and siSTIL EJ cells transfected with siIFT88 or not were respectively detected by western blot analysis. All the percentages of protein levels were quantification. Unpaired t-test analysis was utilized to compare the differences between the two groups. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, ****P-value < 0.0001