Fig. 6

PCTR1 activates CREB by the ALX/PKA pathway to increase GPX4 expression in vitro. BOC-2 (ALX receptor inhibitor, 10 μM), H89 (PKA inhibitor, 10 μM), 666-15 (CREB inhibitor, 1 μM) or an equivalent volume of DMSO was administered to H1299 cells for 30 min in advance, and then LPS and PCTR1 were co-administered for 48 h. A, B The protein level of P- PKA was measured by western blot. C, D The protein level of P-CREB was measured by western blot. E, F The protein level of GPX4 was measured by western blot. Data are presented as the mean ± SD, n = 5–6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns: p > 0.05