Fig. 5
Effects of PCTR1 on LPS-induced ferroptosis in vitro. A H1299 cells were treated with LPS (10 μg/mL) and different concentrations of PCTR1 for 48 h. Fold change in cell viability. B H1299 cells were stimulated with RSL3 (10 nM) and different concentrations of PCTR1 for 48 h. Fold change in cell viability. C–E Representative western blotting and quantification analysis of GPX4 and PTGS2. F–H Relative levels of Fe2+, GSH and MDA. I The level of lipid peroxidation was determined with the C11-BODIPY 581/591 fluorescent probe (original magnification ×400). J Oxidized C11-BODIPY 581/591 probe was quantified by flow cytometry. Data are presented as the mean ± SD, n = 4–6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns: p > 0.05