Fig. 2
Effects of PCTR1 on ferroptosis in LPS-induced ALI. PCTR1 (100 or 200 ng) was injected into the caudal vein of mice 6 h after LPS (15 mg/kg, ip) treatment. All lung specimens were harvested at 24 h after LPS stimulation. A–C Representative western blotting and quantification analysis of GPX4 and PTGS2. D–F Relative values of Fe2+, GSH and MDA concentrations. G Representative TEM images of each group. The black arrow indicates ferroptotic mitochondria. Magnification ×30,000. H Representative H&E staining of lung tissues (original magnification, ×200; inset, ×400). I Acute lung injury score. J–L The relative mRNA expression levels of the inflammatory cytokines: IL-6, TNF-α and IL-1β. The acute lung injury score data are presented as the median and range (25th–75th percentile), and other data are presented as the mean ± SD. n = 4–6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns: p > 0.05. ip: intraperitoneal