Fig. 6

HIGD2A regulate proliferation ability of HCC cells partially by modulating MAPK/ERK signaling pathway. A Volcano plots of differential gene expression in shCtrl and shHIGD2A.1 infected HepG2 cells (data from 3 biological replicates). B Reactome enrichment analysis. Top 10 significantly enriched pathway in shHIGD2A-infected group were shown. C MAPK activation and the protein levels of BCL2L1 were assessed by western blotting in HepG2 and Huh7 cells transfected with shCtrl or shHIGD2A.1 lentivirus. D, E BCL2L1 overexpression alleviated HIGD2A knockdown-mediated growth inhibition and apoptosis in HCC cells measured by CCK8 assay (D) and western blotting (E). Results shown are mean ± SEM. Two-way ANOVA with Tukey multiple comparison test was used for statistical analyses. ***P < 0.001; ns not significant