Fig. 1

HIGD2A expression is elevated in HCC tissue and is correlated with poor prognosis. A–H Relative mRNA levels of HIGD2A and HIGD1A in normal and LIHC tissues from TCGA database (A, E), ICGC database (B, F), GSE14520 (C, G) and GSE64041 (D, H). LIHC, liver hepatocellular carcinoma. I HIGD2A mRNA levels in 24 pairs of HCC and matched peritumor tissues. J HIGD2A protein levels in 24 pairs of HCC and matched peritumor tissues were revealed by western blotting, and the bands were semi-quantified by densitometry and normalized to β-actin. Red indicates pairs with increased HIGD2A in cancer. T tumor, P peritumor. K Kaplan–Meier analysis of OS in HCC patients with high versus low HIGD2A mRNA expression in TCGA database (left) and ICGC database (right). L, M Kaplan–Meier analysis of PFI (L) and DSS (M) in patients with high versus low HIGD2A mRNA expression in TCGA database. Wilcoxon signed ranks test was used in A–J; log-rank tests were used in K–M. *P < 0.05, **P < 0.01 and ***P < 0.001; ns not significant