Fig. 2

Repression of the mTOR signalling pathway reduces DNA methylation in liver cancer cells. A, C IF staining and quantitative analysis of 5mC in SNU423 and SNU449 cells; nuclei were stained with DAPI (scale bar, 2 mm). Cells in the control group were cultured in complete medium, whereas cells in the experimental group were subjected to starvation (cultured in complete medium without serum, glucose, and amino acids) for 24 h. B, D IF staining and quantitative analysis for 5mC in SNU423 and SNU449 cells treated with rapamycin (500 nM) for 24 h. E, F Dot blot analysis of the 5mC levels in SNU423 and SNU449 cells treated with starvation or rapamycin (500 nM) for 24 h. 500 ng, 250 ng and 125 ng meaned the loading amount of cellular DNA