Fig. 9

Improved in vivo anti-tumor effect of 2-BP and PD-1/ PD-L1 blockade cotreatment. A–C Male C57BL/6 mice were injected subcutaneously with 5 × 10⁶ Panc 02 cells, then, the tumor bearing mice were co-treated with 2-BP and anti-PD-1 antibody or IgG isotype control. Schematic representation of experimental plan (A). Tumor volume growth curves (B) and survival curves (C) of the mice bearing Panc 02 tumors with the indicated treatment (n = 6 mice per group). D–F Male C57BL/6 mice were subcutaneously injected with 5 × 10⁶ Panc 02 cells, then, the tumor bearing mice were co-treated with 2-BP and anti-PD-L1 antibody or isotype control IgG. Schematic representation of experimental plan (D). Tumor volume growth curves (E) and survival curves (F) of the mice bearing Panc 02 tumors with the indicated treatment (n = 6 mice per group). Survival curves with time were developed until the animal’s death or the tumor volume reached > 1000 mm³. Differences between survival curves were analyzed using the log-rank (Mantel-Cox) test. Results shown are presented as mean ± SD. (*p < 0.05 and **p < 0.01)